POTENTIAL ACTIVITIES OF ARTEMISIA VULGARIS METHANOL EXTRACT AGAINST NTERA-2 CANCER STEM CELLS

Tóm tắt

Cancer stem cells are the major cause of tumor initiation and metastasis, which can lead to drug resistance and recurrence in cancer patients. The search for natural chemicals that can inhibit the development of cancer stem cells is of interest to many scientists. Artemisia vulgaris L. (in Vietnamese named Ngai cuu) is known for traditional medicine to treat diseases such as diabetes, antiseptic, stomach aches, and cancer prevention. This study aims to evaluate the biological effect of methanol extract from A. vulgaris on the NTERA-2 cancer stem cell line. The activity of the extract was illustrated through cytotoxicity assay using the 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) method on monolayer and three-dimension cell culture models and in vitro migration assay. The extract of A. vulgaris exhibited inhibitory activity on NTERA-2 cells with an IC₅₀ value of 18.31 µg/mL and reduced spheroid formation at the concentration of 500 µg/mL. Besides, this extract significantly reduced cell migration ability at the concentration of IC₅₀ and 2xIC₅₀. Consequently, A. vulgaris crude methanol extract presented obvious effects on NTERA-2 cancer stem cells, providing a new direction for investigating natural compound ingredients of this plant for more efficient cancer treatment.