ANTI-AGGREGATORY AND ANTI-COAGULANT ACTIVITIES OF POLYGONUM CUSPIDATUM EXTRACTS

Tóm tắt

Polygonum cuspidatum, also called Cốt khí củ in Vietnam, is a well-known traditional plant with many approved pharmacological effects. In the current study, various extracts from P. cuspidatum rhizomes were tested for their anti-thrombotic activity on human blood for the first time. The platelet aggregation triggered by collagen was measured in human platelet-rich plasma. The blood clotting time was determined using three factors of the coagulation system: activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT). The results showed that the ethyl acetate extract (PC-EA) displayed the most potent inhibitory effect against collagen-induced platelet aggregation, with the percentage of inhibition increasing from 28.03% at 0.2 mg/mL to 82.38% at 0.8 mg/mL. Moreover, this extract also expressed a more potent anti-coagulant activity than the n-hexane and ethanol extracts (p < 0.05). In addition, PC-EA was the only sample that prolonged the blood clotting time in a dose-dependent manner for both APTT and TT assays. However, this extract could slightly extend the PT at 0.8 mg/mL with 13.57 ± 0.48 s compared to 12.13 ± 0.17 s of DMSO 0.1% used as the negative control (p < 0.05). The ethanol fraction from 0.4 mg/mL possessed considerably higher APTT and TT values than the negative control, meanwhile, the n-hexane display no effects on the blood coagulation by any pathways. In conclusion, the ethyl acetate was the most active fraction from P. cuspidatum. Therefore, this extract could provide a source of promising phytoconstituents used in further prevention and treatment of thrombosis-associated diseases.