QUALITY EVALUATION OF PHELLODENDRON AMURENSE COLLECTED IN VIETNAM USING HPLC-DAD AND QAMS METHODS

Tóm tắt

A simple and sensitive high-performance liquid chromatography (HPLC-DAD) method has been developed to quantify phellodendrine, palmatine chloride, and berberine chloride in Phellodendron amurense collected in Vietnam. Based on this method, a quantitative analysis of multiple components with a single marker (QAMS) was carried out and the relative correction factors (RCFs) were calculated to determine the contents of phellodendrine and palmatine chloride according to berberine chloride (used as an internal standard). The RCF values of phellodendrine and palmatine chloride were 2.79 and 0.30, respectively, according to the internal standard. The accuracy of the QAMS method was verified by comparing it with the results of the external standard method (ESM), as well as the feasibility of the method applied to quality control of Phellodendron amurense. The %RSD of f values in different injection volumes were from 3.76% to 3.98%. The accuracies of QAMS were from 90% to 110% and no significant difference in results was found between the content of phellodendrine and palmatine chloride analyzed by QAMS and external standard method. The findings from QAMS indicated that it was a convenient and precise approach for identifying multiple components, particularly when certain genuine standard substances were inaccessible. It can potentially be utilized for controlling the quality of Phellodendron amurense.