NEUROPROTECTIVE EFFECTS OF TIANMA GOUTENG DECOCTION IN A MIDDLE CEREBRAL ARTERY OCCLUSION MODEL OF ISCHEMIC STROKE

Tóm tắt

This study aimed to evaluate the neuroprotective effects of Tianma Gouteng Decoction (TGD) in a middle cerebral artery occlusion-and-reperfusion (MCAO/R) model. Neurological scores, body weight changes, and cerebral infarct volume were evaluated. In addition, the concentrations of gastrodin, baicalin, 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside (THSG), and geniposide in the TGD extract were analyzed by HPLC to characterize its chemical composition. The results indicated that TGD at 13.7 g/kg (raw herbal materials) significantly attenuated MCAO/R-induced cerebral infarction, improved neurological deficits, and attenuated body weight loss compared with the vehicle-treated group. TGD at 6.8 g/kg (raw herbal materials) also conferred neuroprotective effects, with early functional improvement observed 24 h after reperfusion, whereas TGD at 27.4 g/kg (raw herbal materials) did not produce significant protection. The TGD extract contained gastrodin (1.4 mg/mL), baicalin (10.091 mg/mL), THSG (0.0203 mg/mL), and geniposide (9.023 mg/mL), which may collectively contribute to its pharmacological activity. Overall, these findings demonstrate that TGD exerts neuroprotective effects against transient ischemia-induced brain injury, with optimal efficacy observed at 13.7 g/kg (raw herbal materials), suggesting its potential as a promising therapeutic candidate for ischemic stroke.