DEVELOPMENT AND VALIDATION OF QUANTITATIVE ANALYSIS OF L-DOPA FROM MUCUNA PRURIENS

Tóm tắt

Mucuna pruriens is an important medicinal plant used for the treatment of Parkinson’s disease and many others in ancient traditional Indian medicine. This study reports a simple and rapid high performance liquid chromatography (HPLC) method for the quantification of L-dopa in Mucuna pruriens seeds. Methanol/water/hydrochloric acid (70:30:0.1) was used as the extraction solvent. The chromatographic condition was performed on the Agilent Zorbax Eclipse plus C₁₈ column (250 x 4.6 mm, 5 µm), the mobile phase was 0.1% acetic acid/methanol (99:1), flow rate 0.8 mL/min, and the ultraviolet (UV) detector was set at 280 nm. The method has been validated, showing that it is selective, specific, accurate, and suitable for the quantification of L-dopa in Mucuna seeds. The recovery was 99.89 - 101.49%, and relative standard deviation (RSD) values of the repeatability and intermediate precision were less than 2%. The limit of quantification of L-dopa was 0.12 µg/mL, corresponding to 0.07 mg/g in samples. This method was used to determine the L-dopa content in 5 samples of Mucuna pruriens seeds and showed the L-dopa content ranging from 5.20 ± 0.02% to 5.92 ± 0.10% by weight of dried medicinal herbs.