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METHOD DEVELOPMENT FOR QUANTIFICATION OF PAEDEROSIDIC ACID FROM PAEDERIA SCANDENS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
DOI:
https://doi.org/10.63240/jmm-nimm.2022.2.409Từ khóa:
Paederia scandens (Lour.) Merr., Paederosidic acid, HPLCTóm tắt
A simple and sensitive high performance liquid chromatography (HPLC-DAD) method for quantification of paederosidic acid from Paederia scandens aerial part was developed and validated. The chromatographic separations were obtained by a Shimadzu C18 column (250×4.6mm, 5µm) using isocratic mode with 0.1% phosphoric and acetonitrile (85:15, v/v). Detection was set at a wavelength of 234 nm. The validation results displayed good accuracy, repeatability, linearity, and selectivity. The contents of paederosidic acid in Paederia sc acid andens samples were 0.0075 -0.2339%. The obtained results provide useful information for the quality testing of the Paederia scandens in Vietnam.