CHEMICAL COMPOSITION, ANTI-MICROBIAL, ANTI-INFLAMMATORY, AND CYTOTOXIC ACTIVITIES OF ESSENTIAL OILS FROM MELICOPE PTELEIFOLIA (CHAMP. EX BENTH.) T.G.HARTLEY LEAVES AND STEMS COLLECTED IN LAM DONG PROVINCE

Tóm tắt

The essential oils extracted by hydrodistillation from the leaves and stems of Melicope pteleifolia (MP) collected in Bidoup-Nui Ba National Park, Lam Dong province, Vietnam were investigated for their chemical composition and bioactivity. 29 compounds were identified in the leaf essential oil (MPL), of which trans-α-bergamotene (41.1%), trans-γ-bisabolene (13.0%), linalool (7.6%), and β-atlantol (7.3%) were the most abundant. The stem oil (MPS) contained 21 identified constituents, mainly trans-α-bergamotene (63.7%), limonene (7.4%), and linalool (7.1%). MPL exhibited inhibitory effects on S. aureus, B. subtilis, and L. fermentum, while MPS significantly inhibited the growth of S. aureus, B. subtilis, E. coli, and C. albicans and showed minimal inhibition against three other bacteria. MPL inhibited NO production in macrophage RAW 264.7 cells (IC₅₀ 19.86 ± 0.16 mg/mL). Both MPL and MPS showed moderate cytotoxic effects on SK-LU-1, MCF-7, and HepG2 cell lines with the IC₅₀ values of 20.80 ± 1.42, 25.90 ± 1.47, and 23.42 ± 1.78 mg/mL (for MPL) and 25.36 ± 2.41, 46.46 ± 3.13, and 47.41 ± 2.91 mg/mL (for MPS), respectively. Their antifungal, anti-inflammatory, and cytotoxic activities were evaluated and reported for the first time.