QUANTITATIVE DETERMINATION OF DAPHNORETIN IN AERIAL PARTS OF WIKSTROEMIA INDICA L. (THYMELAEACEAE) BY HPLC-DAD

Tóm tắt

A simple and sensitive HPLC method was proposed for the quantification of daphnoretin in aerial parts of Wikstroemia indica (L.) (W. indica). The chromatographic separations were obtained by an Agilent C₁₈ column (250 x 4.6 mm, 5 µm particle size) using isocratic elution with acetonitrile and water (40/60, v/v), at a flow rate of 0.6 mL/min, with an injection volume of 10 µL and detection wavelength of 345 nm. The calibration curve displayed a good linear relationship (linearity ranging from 14.88 - 238 µg/mL and R²=0.9999). The method was validated for system suitability (RSD of peak area = 0.21%; RSD of t_R = 0.15%); precision (RSD < 3.0%) and accuracy (recovery: 95.60-102.92%). The validation results displayed good accuracy, repeatability, linearity, and selectivity according to the ICH guideline. The percentage content of daphnoretin in aerial part samples of W. indica ranged from 0.010% to 0.207%. The obtained results provided useful information for quality assurance and standardization of W. indica in Vietnam.