ACUTE ORAL TOXICITY AND ANTI-INFLAMMATORY EFFECT OF THE STANDARDIZED EXTRACT FROM VITEX TRIFOLIA L. FRUITS

Tóm tắt

Recent studies suggest that the decline in ovarian function and estrogen levels during menopause cause increased proinflammatory cytokines and oxidative stress associated with these symptoms and perhaps progressive central nervous system (CNS) damage.  Our previous studies have shown that a Vitex trifolia L. n-butanol extract improved cognitive deficit and ameliorated the atrophy of the uterus in ovariectomized mice, as a menopause model. The purpose of this study is to determine acute oral toxicity in mice and evaluate the anti-inflammatory properties of the standardized extract from the fruits of V. trifolia (VTE) collected in Vietnam. The acute toxicity study showed that the VTE had an LD₅₀ value of 26.0 g/kg BW. Our results indicated that in the carrageenan-induced paw edema rat model, VTE at a dose of 150 mg/kg significantly inhibited paw edema at 4 and 6 hours. In addition, VTE at both doses of 150 mg/kg and 300 mg/kg significantly decreased the volume of inflammatory exudate, the number of leukocytes, and the protein content in the exudate in the carrageenan and formaldehyde-induced peritonitis model. Taken together, these results suggest that VTE exhibits potent acute anti-inflammatory effects. Further research is required to assess the potential for preventing or treating CNS-related inflammatory conditions.