HPTLC SCANNER METHOD FOR THE SIMULTANEOUS DETERMINATION OF LYCOPENE AND β-CAROTENE IN GAC SEED ARIL

Tóm tắt

This study successfully established and validated a High-Performance Thin-Layer Chromatography (HPTLC) method, HPTLC Scanner Method for the simultaneous quantification of two marker carotenoid compounds, lycopene and β-carotene, in the aril of Gac (Momordica cochinchinensis Spreng.). This method provides a rapid, cost-effective, and reliable analysis. The validation results, conducted according to international standards (ICH and AOAC), indicated that the new method possesses high reliability, with intra-day and inter-day precision both being below 5%, and the accuracy falling within the acceptable range of 96.50% to 102.12%. The content of the two marker carotenoid compounds in ten samples of Gac seed aril collected in Vietnam revealed significant variability: lycopene ranged from 0.03% to 1.01%, and β-carotene ranged from 0.09 to 1.43 mg/g. The quantification results obtained by the HPTLC-scanner method showed a very high correlation with the previously developed High-Performance Liquid Chromatography (HPLC-DAD) method (R² > 0.95).