STEROID AND TRITERPENOID COMPOUNDS ISOLATED FROM AMANITA PANTHERINA AND THEIR ANTI-INFLAMMATORY ACTIVITY

Tóm tắt

Phytochemical research of the ethanolic extract of Amanita pantherina sensu Gonnermann & Rabenhorst led to the isolation of six steroids (1‒6) and a triterpenoid (7), using various chromatographic separations. Their structures were elucidated to be β-sitosterol (1), stigmasterol (2), cycloeucalenol (3), spinasterol (4), daucosterol (5), stigmasterol 3-O-β-D-glucoside (6), and betulin (7) by detailed analysis of NMR spectroscopic records and comparison with those reported. Compound 7 showed the most inhibitory activity (IC₅₀ = 33.4 μM) against the LPS-induced NO production in macrophage RAW 264.7 cells, followed by compound 3 with an IC₅₀ value of 48.2 μM. Compounds 1, 2, and 4 exhibited weak inhibitory activity with IC₅₀ values of 92.8, 64.9, and 62.4 μM, respectively, while compounds 5 and 6 were inactive (IC₅₀ > 100 μM). This is the first time compounds 3 and 7 have been evaluated for their inhibitory effects on NO production. In addition, compounds 2, 3, and 7 showed weak inhibitory activity against tumor necrosis factor-alpha (TNF-α) with IC₅₀ values of 83.9, 72.8, and 58.1 μM, respectively.