QUANTITATIVE DETERMINATION OF BERBERINE HYDROCHLORIDE IN THE TAN THONG PHONG VISCOUS EXTRACTS BY HPLC

Tóm tắt

An HPLC method was proposed for quantification of berberine hydrochloride in the Tan thong phong viscous extracts (Extractum spissum) which is prepared from Cortex Phellodendri chinensis, Rhizoma Atractylodis, Radix Achyranthis bidentatae, Herba Siegesbeckiae, Rhizoma Anemarrhenae, Fructus Chaenomelis using ethanol and water. The chromatography was established as: Using the Shim-pack GIST C₁₈ column (250 mm × 4.6 mm, 5 µm); The mobile phase includes acetonitrile - 0.05% orthophosphoric acid solution (gradient program); The detection wavelength was 347 nm; Flow rate was 1 mL/min; The column temperature was set at 20^oC; Injection volume was 5 µL. The method was validated for the specificity (RSD of peak area = 0.28%; RSD of t_R = 0.05%); linearity ranging from 11.29 - 361.23 µg/mL (r = 0.9999), precision (RSD < 3.7%) and high accuracy (recovery: 97.77% - 100.36%). The limits of detection (LOD) and quantification (LOQ) were 0.052µg/mL and 0.172 µg/mL, respectively. As for practical application, the tested samples showed the content of berberine hydrochloride was 0.88 ± 0.06%.