ANTIOXIDANT AND XANTHINE OXIDASE INHIBITORY ACTIVITIES OF ENSETE GLAUCUM (ROXB.) CHEESMAN SEEDS

Tóm tắt

Ensete glaucum seeds are commonly known as an herbal traditional medicine with many therapeutic potentials. The purpose of this study was to investigate the antioxidant and xanthine oxidase inhibitory activities of E. glaucum seed extracts as well as the correlation with total polyphenol content (TPC) and total flavonoid content (TFC) using Pearson’s method. The results showed that the 96% ethanol extract of E. glaucum seeds by hot extraction (H96) had higher TPC (67.24 mg GAE/100 mg d. w.) and TFC (8.60 mg QE/g d. w.) than other extracts. E. glaucum seed extracts presented antioxidant property through their DPPH radical scavenging (IC₅₀ of C96 = 5.78 µg/mL), ABTS radical cation decolorization (IC₅₀ of H96 = 10.47 µg/mL) and reducing power (EC₅₀ of H96 = 27.03 µg/mL) abilities. Furthermore, E. glaucum seed extract also showed xanthine oxidase inhibitory activity with the H96 extract having the best effect (IC₅₀ = 76.41 µg/mL). There was a remarkably negative correlation between TPC and TFC in E. glaucum seed extracts with their IC₅₀ of xanthine oxidase, DPPH, ABTS inhibitions, and reducing power activity with correlation coefficient (r) of -0.7877, -0.9392, -0.9290, and -0.9329 (TPC), -0.5620, -0.7664, -0.6150, and -0.6169 (TFC), respectively. It was suggested that E. glaucum seeds have great potential for antioxidant and xanthine oxidase inhibitory activities based on the high total polyphenol and flavonoid contents. Simultaneously, 96% ethanol extract of E. glaucum seeds could be considered as a potential extract for further studies in the direction of antioxidant and anti-hyperuricemic activities.