OPTIMIZATION OF THE EXTRACTION PROCESS FROM YELLOW CAMELLIA FLOWERS (CAMELLIA CUCPHUONGENSIS NINH & ROSMANN) COLLECTED IN PHU THO PROVINCE USING RESPONSE SURFACE METHODOLOGY

Tóm tắt

This study was conducted to optimize the simultaneous extraction of total polyphenol content (TPC) and total flavonoid content (TFC) from the flowers of the yellow Camellia (Camellia cucphuongensis Ninh & Rosmann) collected in Phu Tho province. A Central Composite Design (CCD) combined with Response Surface Methodology (RSM) was employed to evaluate the effects of three independent variables: ethanol concentration (EtOH, 30-70%), extraction temperature (40-80°C), and extraction time (2-8 h). The optimal extraction conditions were identified as 58% EtOH, a temperature of 60°C, and an extraction time of 8 hours. Under these optimized parameters, the experimental values for TFC and TPC were 18.09 ± 0.17% and 35.12 ± 0.28%, respectively, which showed high compatibility with the predicted values. This optimized process provides a reliable scientific basis for the standardized production of bioactive-rich extracts from C. cucphuongensis. These findings highlight the potential of this endemic species as a high-value raw material for the development of antioxidant-based pharmaceuticals and functional foods in the field of natural products chemistry.