EVALUATION OF ANTIOXIDANT AND XANTHINE OXIDASE INHIBITORY ACTIVITY OF DIFFERENT EXTRACTS OF GNETUM MONTANUM MARKGR.

Tóm tắt

To gout patients, finding the natural drugs, which exhibit high safety and good influences with each step of the disease, is trustly important. The in vitro study was performed to investigate DPPH scavenging activity, lipid peroxidation activity and xanthine oxidase inhibitory activity of extracts (aqueous extract, 45% ethanol extract, 80% ethanol extract) from Gnetum montanum stem. In 1,1-diphenyl-2-picrylhydrazyl (DPPH) test, 45% ethanol extract from G. montanum (GM-EE1) (IC₅₀: 6.66 µg/mL) was shown to scavenge DPPH better than 80% ethanol extract from G. montanum (GM-EE2) (IC₅₀: 13.65 µg/mL), aqueous extract from G. montanum (GM-AE) (IC₅₀: 81.85 µg/mL) but it was shown to scavenge DPPH weaker than ascorbic acid (IC₅₀: 4.18 µg/mL). In lipid peroxidation inhibitory activity test, GM-EE1 had the highest antioxidant effect (IC₅₀: 1.18 µg/mL), followed by GM-EE2 (IC₅₀: 1.75 µg/mL), and the lowest was GM-AE (IC₅₀: 10.16 µg/mL). All extracts were shown to lipid peroxidation inhibitory activity better than Trolox (IC₅₀: 32.07 µg/mL). The evaluation of xanthine oxidase inhibition shows that GM-EE1 had the strongest xanthine oxidase inhibitory activity (IC₅₀: 1.79 µg/mL), followed by GM-EE2 (IC₅₀: 2.21 µg/mL), and the lowest was GM-AE (IC₅₀: 17.83 µg/mL). These results indicate that 45% ethanol extract of G. montanum was good antioxidant and moderate type xanthine oxidase inhibitor.